| Объем | 100 мкл |
| Синонимы | Angiomotin, AMOT, KIAA1071 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q4VCS5 |
| Иммуноген | Recombinant Human Angiomotin protein (1-300AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
| Примечание | Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cancer, Cardiovascular, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Angiomotin antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 119, 73 kDa
Observed band size: 119 kDa
 |
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: AMOT antibody at 1.21µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 119 kDa
Observed band size: 130 kDa
 |
IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 |
IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 |
| | |
Наименование: AMOT Antibody.