6×His Tag AntibodyСпецификация| Объем | 50 мкл | | Синонимы | HHHHHH Tag | | Клональность | Polyclonal Antibody | | Иммуноген | Synthetic peptide: 18×His conjugated to KLH | | Источник | Rabbit | | Видовая специфичность | HIS Tag | | Применение | ELISA, WB,ELISA:1:5000-1:10000, WB:1:2000-1:10000 | | Примечание | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen affinity purification | | Области исследований | Others | | Ссылка на страницу на сайте производителя | ссылка | Gel: 8%SDS-PAGE, Lysate: 0.5 ?g, Lane: Recombinant 6HIS Tagged protein , Primary antibody: CSB-PA270215(6His Antibody) at dilution 1/2000 (lane 1),1/5000 (lane 2),1/8000 (lane 3),1/10000 (lane 4), Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
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Наименование: 6×His Tag Antibody.