| Объем | 100 мкг |
| Синонимы | Hypoxia-inducible factor 1-alpha (HIF-1-alpha) (HIF1-alpha) (ARNT-interacting protein) (Basic-helix-loop-helix-PAS protein MOP1) (Class E basic helix-loop-helix protein 78) (bHLHe78) (Member of PAS protein 1) (PAS domain-containing protein 8), HIF1A, BHLHE78 MOP1 PASD8 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q16665 |
| Иммуноген | Recombinant Human Hypoxia-inducible factor 1-alpha protein (579-826AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Hypoxia-inducible factor 1-alpha antibody at 2µg/ml
Lane 1: HGC27 whole cell lysate
Lane 2: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 93, 83, 96 kDa
Observed band size: 93 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA12807A0Rb at dilution of 1:50
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Immunocytochemistry analysis of human bladder cancer using CSB-PA12807A0Rb at dilution of 1:100
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Immunocytochemistry analysis of human colon cancer using CSB-PA12807A0Rb at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA12807A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: Rabbit anti-humanHypoxia-inducible factor 1-alphapolyclonal Antibody.