| Объем | 100 мкг |
| Синонимы | T-cell surface glycoprotein CD3 epsilon chain (T-cell surface antigen T3/Leu-4 epsilon chain) (CD antigen CD3e), CD3E, T3E |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P07766 |
| Иммуноген | Recombinant Human T-cell surface glycoprotein CD3 epsilon chain protein (23-207AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | The CD3 complex mediates signal transduction. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | T-cell surface glycoprotein CD3 epsilon chain protein |
| Области исследований | Immunology, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CD3E antibody at 1.5µg/ml + Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
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IHC image of CSB-PA10484A0Rb diluted at 1:80 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA10484A0Rb diluted at 1:80 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of PC3 cells with CSB-PA10484A0Rb at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: Антитела Rabbit anti-human T-cell surface glycoprotein CD3 epsilon chain polyclonal Antibody / Rabbit anti-human T-cell surface glycoprotein CD3 epsilon chain polyclonal Antibody.