| Объем | 100 мкг |
| Синонимы | Glucose-6-phosphate isomerase (GPI) (EC 5.3.1.9) (Autocrine motility factor) (AMF) (Neuroleukin) (NLK) (Phosphoglucose isomerase) (PGI) (Phosphohexose isomerase) (PHI) (Sperm antigen 36) (SA-36), GPI |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P06744 |
| Иммуноген | Recombinant Human Glucose-6-phosphate isomerase (2-554AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Cancer, Immunology, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00367A0Rb at dilution of 1:100
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Western Blot
Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Rat brain tissue, Mouse heart tissue, Mouse liver tissue, Mouse kidney tissue, Mouse brain tissue
All lanes: GPI antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00367A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA00367A0Rb at dilution of 1:100
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Immunofluorescent analysis of PC-3 cells using CSB-PA00367A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating GPI in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA00367A0Rb in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA00367A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: Антитела Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody / Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody.