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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

Антитела Rabbit anti- human Proteasome subunit beta type-4 polyclonal Antibody

Rabbit anti- human Proteasome subunit beta type-4 polyclonal Antibody

Immunogen: Recombinant human Proteasome subunit beta type-4 protein
Raised in: Rabbit
Species Reactivity: Human
Tested applications: ELISA, WB, IHC
Application notes:
Relevance: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Clonality: Polyclonal
Isotype: IgG
Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
Conjugate: Non-conjugated
Stroage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form: Liquid
Stroage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
References: [1] "Sequence analyses and inter-species comparisons of three novel human proteasomal subunits, HsN3, HsC7-I and HsC10-II, confine potential proteolytic active-site residues."Nothwang H.G., Tamura T., Tanaka K., Ichihara A.Biochim. Biophys. Acta 1219:361-368(1994) [2] "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.Nat. Genet. 36:40-45(2004) [3] "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Images:
Western blot
Proteasome subunit beta type-4 Antibody at 2 µg/ml + EC109 whole cell lysate at 20 µg
 
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size : 29 kDa
Observed band size : 75 kDa
 
Why is the actual band size different from the predicted?
Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...
·         post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein
·         post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
·         splice variants - alternative splicing may create different sized proteins from the same gene
·         relative charge - the composition of amino acids (charged vs non-charged)
multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.

Alias : 26 kDa prosomal protein HsBPROS26 PROS-26 Macropain beta chain Multicatalytic endopeptidase complex beta chain Proteasome chain 3 HsN3.

Информация для заказа

Область использования:Производство:Cusabio
Метод: 
Объем:100 мкг 
Кат. номер:CSB-PA01375A0Rb
Цена (с НДС 20%):по запросуВ корзину
Антитела Rabbit anti- human Proteasome subunit beta type-4 polyclonal Antibody / Rabbit anti- human Proteasome subunit beta type-4 polyclonal AntibodyНаименование: Антитела Rabbit anti- human Proteasome subunit beta type-4 polyclonal Antibody / Rabbit anti- human Proteasome subunit beta type-4 polyclonal Antibody.
Примечание: дополнительная информация (на английском языке).
   
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