АнтителаAnti-CD11b antibody [CD11b]

Product nameAnti-CD11b antibody [CD11b]
See all CD11b primary antibodies
Description
Mouse monoclonal [CD11b] to CD11b
Tested applicationsSuitable for: ICC/IF, IHC - Wholemount, Flow Cytmore details
Species reactivity
Reacts with: Rat
Immunogen
Tissue, cells or virus corresponding to Rat CD11b. Immunogen is rat neutrophils.

Our Abpromise guarantee covers the use of ab8879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		Use at an assay dependent concentration.
IHC - Wholemount		Use at an assay dependent concentration.
Flow Cyt		Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localizationMembrane.
Information by UniProt
Database links
- Entrez Gene: 25021 Rat
- Unigene: 54465 Rat
Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
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IHC - Wholemount - Anti-CD11b [CD11b] antibody (ab8879)This image is courtesy of an anonymous Abreview

IHC - Wholemount of rat retina tissue labelling CD11b (white) with ab8879. Sample was incubated with primary antibody (1/100 in PBS) for 24 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal (1/1000) was used as the secondary antibody. Blue - DAPI.

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [CD11b] (ab8879)This image is courtesy of an anonymous Abreview

ab8879 staining CD11b in mixed rat spinal cord cultures on astrocytes by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were live stained. Samples were incubated with primary antibody (1/200 in DMEM) for 20 minutes. An Alexa Fluor®488-conjugated Rat anti-rabbit IgG1 monoclonal (1/600) was used as the secondary antibody.

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Flow Cytometry - Anti-CD11b antibody [CD11b] (ab8879)

NR8383 cells, rat macrophage cells, were used for a flow cytometry analysis with ab8879 (CD11b) antibody that binds the rat Mac-1 alpha subunit (CD11b). Cells were treated with ab8879 at a 1:50 dilution and subsequently with anti-murine IgG FITC. A 94% shift is seen as compared to the non-treated control.

Liu J et al. HIV-1 Tat protein increases microglial outward K(+) current and resultant neurotoxic activity. PLoS One 8:e64904 (2013). ICC/IF ; Rat . Read more (PubMed: 23738010) »
Rui YF et al. Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy. Stem Cells Dev 22:1076-85 (2013). Flow Cyt ; Rat . Read more (PubMed: 23106341) »

Область использования: Антитела Abcam	Производство:	Abcam
	Метод:
	Объем:	100 мкг
	Кат. номер:	ab8879
Цена (с НДС 20%):	по запросу	В корзину
Наименование: АнтителаAnti-CD11b antibody [CD11b]. Примечание: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Дополнительная информация (на английском).