The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution. IHC-Fr: 1/100. 2% paraformaldehyde-lysine / sodium periodate or acetone fixation.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
Cellular localizationCell Membrane
References for Anti-pan Macrophage antibody [RM-1] (ab15615)
This product has been referenced in:
Kasper M et al. Heterogeneous Dolichos biflorus lectin binding to a subset of rat alveolar macrophages in normal and fibrotic lung tissue. Acta Histochem95:1-11 (1993). Read more (PubMed: 8279229) »
Izumi S et al. Ontogenetic development of synovial A cells in fetal and neonatal rat knee joints. Cell Tissue Res262:1-8 (1990). Read more (PubMed: 2257604) »