Anti-pan Macrophage antibody [RM-1]

• Product nameAnti-pan Macrophage antibody [RM-1]
• Description
  Mouse monoclonal [RM-1] to pan Macrophage
• Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
• Species reactivity
  Reacts with: Rat
• Immunogen

  Tissue/ cell preparation (Rat).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: PBS, 10mg/ml BSA. pH 7.2
• Concentration50 µg at 0.2 mg/ml 
  
• ClonalityMonoclonal
• Clone numberRM-1
• IsotypeIgG1
• Research areas
  • Immunology
   
  • Innate Immunity
   
  • Macrophage / Inflamm.

  • Neuroscience
   
  • Cell Type Marker
   
  • Glia marker
   
  • Microglia marker

Applications

• Application notesFlow Cyt: Use at an assay dependent dilution. 
  IHC-Fr: 1/100. 2% paraformaldehyde-lysine / sodium periodate or acetone fixation. 
  
  Not tested in other applications. 
  Optimal dilutions/concentrations should be determined by the end user.

Target

• RelevanceThe antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
• Cellular localizationCell Membrane