Антитела Anti-CD8 alpha antibody [OX-8], мышиные, моноклональные

Anti-CD8 alpha antibody [OX-8]

Product nameAnti-CD8 alpha antibody [OX-8]
See all CD8 alpha primary antibodies
Description
Mouse monoclonal [OX-8] to CD8 alpha
Tested applicationsBlocking, Flow Cyt, IHC-Fr, IHC-P, WB, IP, ICC/IFmore details
Species reactivity
Reacts with: Rat
Immunogen
Rat thymocyte membrane glycoproteins.

FormLiquid
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Concentration125 µg at 1 mg/ml
PurityProtein G purified
ClonalityMonoclonal
Clone numberOX-8
MyelomaNS1
IsotypeIgG1
Research areas

Our Abpromise guarantee covers the use of ab33786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Blocking		Use at an assay dependent concentration.
Flow Cyt		1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration.
IP		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.

FunctionIdentifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Involvement in diseaseDefects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
Cellular localizationSecreted and Cell membrane.
Information by UniProt
Database links
- Entrez Gene: 24930 Rat
- SwissProt: P07725 Rat
- Unigene: 10306 Rat
Alternative names
- alpha polypeptide (p32) antibody
- CD8 antibody
- CD8 antigen alpha polypeptide antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)Image courtesy of an anonymous Abreview.

ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with 10% neutral buffered formalin and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then permeabilized with Triton X-100, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab33786 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a biotin-conjugated rabbit anti-mouse polyclonal, used at a 1/300 dilution.
See Abreview
Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [OX-8] (ab33786)Image courtesy of an anonymous Abreview.

ab33786 staining CD8 alpha in rat spleen tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in acetone, blocked with 5% serum for 30 minutes at 25°C, then incubated with ab33786 at a 1/200 for 1 hour 30 minutes at 25°C. The secondary used was a biotin conjugated rabbit anti-mouse polyclonal, used at a 1/300.
See Abreview

Muhamed J et al. Comparative local immunogenic potential of scaffolds prepared from porcine cholecyst, jejunum, and urinary bladder in rat subcutaneous model. J Biomed Mater Res B Appl Biomater N/A:N/A (2014). Read more (PubMed: 25370716) »
Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »

Область использования: Прочие реагенты для иммуногистохимии	Производство:	Abcam
	Метод:
	Объем:	125 мкг
	Кат. номер:	ab33786
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Anti-CD8 alpha antibody [OX-8], мышиные, моноклональные / Anti-CD8 alpha antibody [OX-8]. Примечание: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Дополнительная информация (на английском).