Антитела Anti-CD4 [OX-35], мышинные, моноклональные

Anti-CD4 antibody [OX-35]

Product nameAnti-CD4 antibody [OX-35]
See all CD4 primary antibodies
Description
Mouse monoclonal [OX-35] to CD4
Tested applicationsICC, Flow Cyt, IHC-Frmore details
Species reactivity
Reacts with: Rat
Immunogen
MLR generated rat T helper lymphocytes.
EpitopeThis antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal (eg ab33779 and ab33783).

FormLiquid
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration100 µg at 1 mg/ml
PurityProtein G purified
ClonalityMonoclonal
Clone numberOX-35
MyelomaNS1
IsotypeIgG2a
Research areas

Our Abpromise guarantee covers the use of ab33775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC		Use at an assay dependent concentration.
Flow Cyt		1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr		Use at an assay dependent concentration.

FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Information by UniProt
Database links
- Entrez Gene: 24932 Rat
- SwissProt: P05540 Rat
- Unigene: 10748 Rat
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all

Thampi P et al. Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation. Artif Organs 37:600-5 (2013). IHC ; Rat . Read more (PubMed: 23452255) »
Pohlers D et al. Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on NF-kappaB binding activity and TNF-alpha secretion of T cells. Arthritis Res 4:184-9 (2002). Read more (PubMed: 12010568) »

Область использования: Прочие реагенты для иммуногистохимии	Производство:	Abcam
	Метод:
	Объем:	100 мкг
	Кат. номер:	ab33775
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Anti-CD4 [OX-35], мышинные, моноклональные / Anti-CD4 antibody [OX-35]. Примечание: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Дополнительная информация (на английском).