ООО «Лабораторная Диагностика»
ООО «Лабораторная Диагностика» info@LD.ru    тел.: +7 495 369-20-43
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/ Каталог / Иммунология / Иммуногистохимия / Микроскопы. Микроскопическая техника / ELECTRON MICROSCOPY SCIENCES - Продукты для гистологии, световой микроскопии и электронной микроскопии

Гистология и световая микроскопия

Теперь мы предлагаем полную линейку подготовленных, готовых к использованию высоко качественных окрашивающих растворов для всех стандартных процедур окрашивания.

  • Все растворы и концентраты упакованы и приготовлены в удобных и точных количествах.
  • Готовые к употреблению смеси; экономят ваше время и силы
  • Получаете стабильно однородные результаты

arrow11Acid Fuchsin Aqueous Solutions

RT 26020-02 Acid Fuchsin, 0.2% Aqueous msds 500 ml
RT 26020-05 Acid Fuchsin, 0.5% Aqueous msds 500 ml
RT 26020-10 Acid Fuchsin, 1.0% Aqueous msds 500 ml
RT 26020-11 Acid Fuchsin, 1.0% in 1% Acetic Acid msds 500 ml

arrow11Acridine Orange Aqueous Solution

RT 26024-01 Acridine Orange 0.1% Aqueous msds 500 ml
RT 26024-02 Acridine Orange 0.2% Aqueous msds 500 ml

arrow11Alcian Blue Solution

RT 26026-01 Alcian Blue, 1% Aqueous msds 250 ml
RT 26026-11 Alcian Blue, 1% in 1% Acetic Acid msds 250 ml
RT 26026-13 Alcian Blue, 1% in 3% Acetic Acid, pH 2.5 msds 250 ml

arrow11Alcoholic Stain Solutions

RT 26090-05 Alcoholic Congo Red, 0.2% Solution msds 500 ml
RT 26091-01 Alcoholic Hematoxylin, 1% in 95% Alcohol msds 500 ml
RT 26091-10 Alcoholic Hematoxylin, 10% in 95% Alcohol msds 500 ml
RT 26092-05 Alcoholic Iodine, 0.5% in 95% Alcohol msds 500 ml
RT 26092-06 Alcoholic Iodine, 0.5% in 85% Alcohol msds 500 ml
RT 26092-20 Alcoholic Iodine, 2% in 85% Alcohol msds 500 ml
RT 26093-60 Alcoholic Saffron, 6% msds 100 ml
RT 26094-05 Alcoholic Thionin, 0.5% in 20% Alcohol msds 500 ml
RT 26096 Aldehyde Fuchsin Solution msds 250 ml
RT 26097 Alum Hematoxylin Solution msds 500 ml

msdsarrow11Alum Celestine Blue

(Iron Alum – Celestine Blue)
Lendrum & McFarlane, (1940). J. of Pathology & Bacteriology, v. 50, pp 381
A stain solution for nuclei. A substitute for Hematoxylin/Eosin.

RT 26023-02 Alum Celestine Solution 250 ml
RT 26023-05 Alum Celestine Solution 500 ml

arrow11Alum Hematoxylin Solution

For staining nuclei

RT 26097 Alum Hematoxylin Solution 500 ml 40.00 Add to Cart

arrow11Aniline Blue Solution

RT 26027-10 Aniline Blue, 2.5% in 2% Acetic Acid msds 500 ml
RT 26027-20 Aniline Blue-Phosphomolybdic Acid Solution msds 500 ml
RT 26027-30 Aniline Blue-Orange G Solution msds 500 ml

arrow11Basic Fuchsin Solution

RT 26031-02 Basic Fuchsin 0.25% Aqueous msds 500 ml
RT 26031-05 Basic Fuchsin 0.5% Aqueous msds 500 ml
RT 26031-10 Basic Fuchsin 0.1% in 95% Methanol msds 500 ml
RT 26031-20 Basic Fuchsin Working Solution msds 500 ml

msdsarrow11Best's Carmine Stock Solution

Stain is used for selectively staining glycogen. (Keep Refrigerated)

RT 26025-02 Best's Carmine Stain Solution 250 ml
RT 26025-05 Best's Carmine Stain Solution 500 ml

 

arrow11Biebrich Scarlet Solution

RT 26033-02 Biebrich Scarlet, 0.2% in 1% Acetic Acid msds 500 ml
RT 26033-10 Biebrich Scarlet, 1% in 1% Acetic Acid msds 500 ml
RT 26033-20 Biebrich Scarlet, 1% Aqueous msds 500 ml
RT 26033-25 Biebrich Scarlet – Acid Fuchsin msds 500 ml

arrow11Congo Red Solution

RT 26090-05 Alcoholic Congo Red, 0.2% Solution msds 500 ml
RT 26090-20 Congo Red, 0.5% Aqueous msds 500 ml
RT 26090-25 Congo Red, 1% Aqueous msds 500 ml

arrow11Cresyl Violet Acetate Solution

RT 26089-01 Cresyl Violet Acetate, 0.1% Aqueous msds 500 ml
RT 26089-20 Cresyl Violet Acetate, 2% Aqueous msds 250 ml

arrow11Crystal Violet Solution

RT 26088-10 Crystal Violet, 1% Aqueous msds 500 ml
RT 26088-15 Crystal Violet, Hucker's Solution msds 500 ml
RT 26088-16 Crystal Violet, Lieb's Stock Solution   250 ml
RT 26088-17 Crystal Violet, Lilies Solution msds 250 ml
RT 26088-18 Crystal Violet, Stirling's Solution   250 ml

arrow11Differential Quick Staining Kit (Modified Giemsa)

msdsmsdsmsds

This kit is a Modified Giemsa Stain kit for quick turn around results. The kit may be used for rapid blood smears for differential assessment, as well as for the detection of H. Pylori microorganisms. The kit comes complete with a fixative for air dried cell suspensions and or touch preparation slides. Applications include fine needle aspirations, frozen sections, blood smears, cytological specimens, cytopreps, microorganism detection and bone marrow biopsies. This stain offers results in 15 seconds. The Kit comes complete with 3 solutions and is available in 3 sizes.

RT 26096-25 Differential Quick Staining Kit 250 ml
RT 26096-50 Differential Quick Staining Kit 500 ml
RT 26096-75 Differential Quick Staining Kit 1 gal

msdsarrow11EA Counterstain, Gill's Modified Solution

For use with Papanicolaou stain; a general tissue stain. Ready-to-use.

RT 26050-05 EA Counterstain, Gill's Modified 500 ml
RT 26050-06 EA Counterstain, Gill's Modified 1 L

msdsarrow11Ehrlich's Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT 26040-05 Ehrlich's Hematoxylin 500 ml
RT 26040-06 Ehrlich's Hematoxylin 1 L

Training ManualTraining ManualEMS Rapid Pro TDSEMS Rapid Pro Processing Systemarrow11

EMS Rapid Pro Processing System

A Revolutionary Rapid Tissue Processing System

Tissue processing has not changed in more than a century... until now. The EMS Rapid Pro Processing System can cut total processing time to as little as 46 minutes.

Advantages over traditional processing

  • Speed – no other processing chemicals will deliver results this fast
  • Increased throughput
  • Suitable for both fresh and frozen sections
  • Catalyzed versions of standard processing chemicals eliminate the need for new safety features, storage, or learning curves
  • Effective for manual or automatic tissue processing
  • Compatible with existing tissue processing equipment
  • "Results While You Wait" option for patients

For more staining protocols, see the Technical Data Sheet.

A proven and reliable method for obtaining rapid results in tissue processing

Process Standard Processing Time EMS Rapid Pro Processing Time
Formalin ½ hr - 1 hr 3 min
70% Ethanol 1 hr 3 min
95% Ethanol 1 hr 3 min
98% Ethanol 1 hr 3 min
90% Xylenes ½ hr - 1 hr 3 min
98% Ethanol 1 hr 3 min
90% Xylenes ½ hr - 1 hr 3 min
Paraffin 2 - 3 hrs 25 min
Total Processing TIme 7½ - 10 hrs 46 min

Ordering

26750-10 EMS Rapid ProKit    
26750-01 EMS Rapid Pro Solution 1 msds 250 ml
26750-02 EMS Rapid Pro Solution 2 msds 250 ml
26750-03 EMS Rapid Pro Solution 3 msds 250 ml
26750-04 EMS Rapid Pro Solution 4 msds 250 ml
26750-05 EMS Rapid Pro Solution 5 msds 250 ml

arrow11Eosin Y Solution

RT 26051-01 Eosin Y, 0.1% Aqueous msds 500 ml
RT 26051-10 Eosin Y, 1% Aqueous msds 500 ml
RT 26051-11 Eosin Y, 1% Alcoholic msds 500 ml
RT 26051-20 Eosin Y – Eosin B Solution msds 500 ml
RT 26051-21 Eosin Y – Phloxine B Solution msds 500 ml

arrow11Fast Green FCF Solution

RT 26053-02 Fast Green FCF, 0.2% Aqueous msds 500 ml
RT 26053-05 Fast Green FCF, 0.5% Aqueous msds 500 ml
RT 26053-25 Fast Green FCF, 2.5% in 2% Acetic Acid msds 500 ml

technical data sheetmsdstechnical data sheetarrow11

Fluorophore-Infiltrated Resin Microscopy (FIRM)

Submit a follow up request

Rapid staining technique that provides high-contrast fluorescence microscopy imaging of resin embedded samples, reminiscint of low magnification transmission electron microscopy.

  • Works with Epoxy and Acrylic type resins
  • Simply mount your sections on glass slides, air dry, and overlay with 100 microliters FIRM for 30 seconds, wash and coverslip with water or an aqueous mountant. View in Rhodamine channel of a standard wide field Fluorescence Microscope.
  • FIRM staining is unique in that the brightly fluorescent dye infiltrates the resin, revealing tissue structures in negative relief, with extremely high contrast and resolution.
  • Perfect for previewing specimens prior to EM Analysis
  • High Throughput, high contrast image phenotyping of model organisms such as drosophila
  • Ideal for material and food science analytical microscopy
RT 12780-05 FIRM for Acrylic Resin Sections msds 10 ml
RT 12780-06 FIRM for Epoxy Resin Sections msds 10 ml

msdsTechnical Data Sheetarrow11Giemsa Solution, C.N.#DcGe-19

Ingredients:

Glycerin (cas# 56-81-5):   61%
Methyl Alcohol (cas# 67-56-1):   38.3%
Azur II (cas# 37247-10-2) :   0.16%
Azur II Eosin (cas# 53092-85-6):   0.6%

Specific Gravity: 0.89

Differentiates types of leucocytes, bacteria and inclusion bodies.

Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films; shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929) Bone marrow and stain for showing cell morphology; together with May-Grunwald Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)

RT 15940 Giemsa Stock Solution 100 ml
RT 15941 Giemsa Stock Solution 500 ml

msdsarrow11Giemsa Stain, C.N.#DcGe-19, Powder

CAS #51811-82-6

RT 15942   10 g

arrow11Giemsa for Thin Film and Giemsa for Thick Film

See listing in Prepared Staining Kit Section.

arrow11Giemsa for Differential Assessment

See listing above for Differential Quick Staining Kit (Modified Giemsa)

arrow11Gill's Hematoxylin Staining Solution

Gill's Hematoxylin is a specially formulated solution for use as a biological stain for the nuclei and nuclear chromatin of normal and abnormal cells whether in whole or sliced cells. Our Gill's Hematoxylin is designed to use at its full strength. It is microfiltered prior to shipment; therefore, filtration before use is not required. For repeated use further filtration is recommended. At the discretion of the user, the Gill's Hematoxylin No. 2 and No. 3 may be diluted with ethylene glycol (25% is recommended) to produce a weaker stain solution.

RT 26030-10 Gill's Hematoxylin No. 1 msds 1 L
RT 26030-14 Gill's Hematoxylin No. 1 4 L
RT 26030-20 Gill's Hematoxylin No. 2 msds 1 L
RT 26030-24 Gill's Hematoxylin No. 2 4 L
RT 26030-30 Gill's Hematoxylin No. 3 msds 1 L
RT 26030-34 Gill's Hematoxylin No. 3 4 L

msdsarrow11Goodpasture's Stain Solution (MacCallum)

RT 26031 Goodpasture's Stain Solution 500 ml

msdsarrow11Gram's Iodine Solution

For use as a bacterial, fungal and inclusion body stain.

RT 26054-05 Gram's Iodine Solution 500 ml
RT 26054-06 Gram's Iodine Solution 1 L

technical data sheetarrow11Harris' Hematoxylin

Stain selectivity for nuclei, fat and lipids. Ready-to-use.

RT 26041-05 Harris' Hematoxylin msds 500 ml
RT 26041-06 Harris' Hematoxylin 1 L
RT 26041-20 Harris' Hematoxylin without Mercury msds 500 ml

arrow11Hematoxylin Solution

RT 26042-1 Hematoxylin, 1% Aqueous msds 500 ml
RT 26042-5 Hematoxylin, 5% in SD Alcohol msds 500 ml
RT 26042-10 Hematoxylin in 95% Alcohol   500 ml
RT 26042-20 Heidenhain's Hematoxylin msds 500 ml

msdsarrow11ImmunoSaver Antigen Retriever

Immunosaver allows for Immunostaining with quick and easy activation cell membranes and the nucleus. Immunosaver provides efficient antigen retrieval for successful immunostaining of a wide variety of antigens under optimized conditions. Protocols for both Light and Electron Microscopy may be found with this reagent.

RT 64142 ImmunoSaver 100 ml

msdstechnical data sheetarrow11Jenner Stain Solution

RT 26033-05 Jenner Stain Solution 500 ml

msdstechnical data sheetarrow11Kinyoun's Solution

Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.

RT 26087-05 Kinyoun's Solution 500 ml
RT 26087-06 Kinyoun's Solution 1 L

msdsmsdsarrow11Leishman's Stain

RT 26086-05 Leishman's Stain 500 ml
RT 26086-50 Leishman's Working Buffer 500 ml

msdsmsdsarrow11Light Green SF Yellowish Solution

RT 26085-05 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
500 ml
RT 26085-06 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
1 L
RT 26085-10 Light Green SF Yellowish,
1% Aqueous
500 ml

msdsarrow11Lissamine Fast Red Solution

RT 26034-05 Lissamine Fast Red Solution 500 ml

msdsarrow11Lugol's Iodine

For use as a bacterial, fungal and inclusion body stain.

RT 26055-05 Lugol's Iodine Solution 500 ml
RT 26055-06 Lugol's Iodine Solution 1L

msdsmsdsarrow11Luxol Fast Blue Solution

RT 26056-10 Luxol Fast Blue, 0.1% in 95% Alcohol
w/Acetic Acid
500 ml
RT 26056-15 Luxol Fast Blue, 0.1% in 95% Alcohol 500 ml

msdsarrow11MacFarlane's Solution

RT 18035-05 MacFarlane's Stock Solution 500 ml
RT 18036-05 MacFarlane's Working Solution 500 ml

msdsarrow11Mayer's Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT 26043-05 Mayer's Hematoxylin 500 ml
RT 26043-06 Mayer's Hematoxylin 1 L
RT 26043-10 Mayer's Hematoxylin 4 L

msdsarrow11Modified Mayer's Hematoxylin

Stock Solution. Ready-to-use.
Ingredients: Hematoxylin 0.4%, Ammonium Aluminum Sulfate 0.5%,
Sodium Iodate: 0.03%, Citric Acid 0.1%, Chloral Hydrate 5%

RT 26043-18 Modified Mayer's Hematoxylin 500 ml

msdsarrow11May-Grunwald Staining Solution

A ready-to-use stain for bone marrow staining.

RT 26064-05 May-Grunwald Stain Solution 500 ml
RT 26064-06 May-Grunwald Stain Solution 1 L

msdsarrow11Methenamine Solution

A stock solution for use in bacterial and fungal stains (Grocott's Method/GMS).

RT 26083-05 Methenamine, 3% Aqueous Solution 500 ml
RT 26083-06 Methenamine, 3% Aqueous Solution 1 L

arrow11Methylene Blue Solution

RT 26075-10 Methylene Blue, 1% Aqueous msds 500 ml
RT 26075-14 Methylene Blue, 1.4% in 95% Alcohol msds 500 ml
RT 26075-20 Methylene Blue, Working Solution msds 500 ml

msdsarrow11Methyl Green-Pyronin, Purified Aqueous Solution

A stain solution is for demonstrate RNA and DNA.

RT 18710-02 Methyl Green-Pyronin Solution 250 ml
RT 18710-05 Methyl Green-Pyronin Solution 500 ml

msdsarrow11Miller's Elastic Stain

RT 26076-05 Miller's Elastic Stain 500 ml
RT 26076-10 Miller's Elastic Stain 1 L

msdsarrow11Mucicarmine

For use with hematoxylin and metanil yellow for the determination of carbohydrates and mucosubstances.

RT 26080-05 Mucicarmine Stock Solution 500 ml
RT 26080-06 Mucicarmine Stock Solution 1 L

msdsmsdsarrow11New Methylene Blue Solution

RT 26077-05 New Methylene Blue 0.50% Aqueous 500 ml
RT 26077-20 New Methylene Blue Solution (Reticulocyte Stain) 500 ml

msdsarrow11Nuclear Fast Red Solution

RT 26078-05 Nuclear Fast Red Solution 500 ml

msdsmsdsarrow11Oil Red O Solution

RT 26079-05 Oil Red O Saturated in Isopropanol 500 ml
RT 26079-15 Oil Red O Saturated in Propylene Glycol 500 ml

msdsarrow11Orange G Solution

RT 26066-05 Orange G, 0.5% Aqueous 500 ml
RT 26066-15 Orange G-Phosphotungstic Acid Solution 500 ml

msdsarrow11Papanicolaou Stain, OG-6

RT 26048-05 OG-6 Stain Solution 500 ml
RT 26048-06 OG-6 Stain Solution 1 L

msdsarrow11Papanicolaou Stain, EA-50 (EA Counterstain)

RT 26049-05 EA-50 Stain Solution 500 ml
RT 26049-06 EA-50 Stain Solution 1 L

msdsarrow11Resorcin-Fuchsin Solution

RT 26067-05 Resorcin-Fuchsin Working Solution 500 ml

arrow11Rosin Alcohol Solution

RT 26068-05 Rosin Alcohol Stock Solution 500 ml
RT 26068-15 Rosin Alcohol Working Solution 500 ml

msdsmsdsarrow11Safranin O Solution

RT 26069-05 Safranin O, 0.1% in 0.1% Acetic Acid 500 ml
RT 26069-15 Safranin O, 0.2% in 1% Acetic Acid 500 ml

msdsmsdsarrow11Toluidine Blue O Solution

RT 26074-05 Toluidine Blue O , 0.1% Aqueous Solution 500 ml
RT 26074-15 Toluidine Blue O, 1% Aqueous Solution 500 ml

msdstechnical data sheetarrow11Van Gieson's Solution

To use with Weigert's Iodine or Weigert's Iron as a connective tissue stain.

RT 26046-05 Van Gieson's Solution 500 ml
RT 26046-06 Van Gieson's Solution 1 L

msdsarrow11Weigert's Iodine Solution

A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases

RT 26045-05 Weigert's Iodine Solution 500 ml
RT 26045-06 Weigert's Iodine Solution 1 L

msdsmsds

Weigert's Iron Hematoxylin

Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.

RT 26044-05 Weigert's Iron Hematoxylin A 500 ml
RT 26044-06 Weigert's Iron Hematoxylin A 1 L
RT 26044-15 Weigert's Iron Hematoxylin B 500 ml
RT 26044-16 Weigert's Iron Hematoxylin B 1 L

msds

arrow11Woodstain Scarlet-Acid Fuchsin Solution

RT 26061-05 Woodstain Scarlet-Acid Fuchsin Solution 500 ml

msdstechnical data sheetarrow11Wright Staining Solution

For use as a blood stain. Ready-to-use.

RT 26060-05 Wright Stain Solution 500 ml
RT 26060-06 Wright Stain Solution 1 L

msdsmsdsmsdstechnical data sheet

arrow11Differential Quick Staining Kit (Modified Giemsa)

This kit is a Modified Giemsa Stain kit for quick turn around results. The kit may be used for rapid blood smears for differential assessment, as well as for the detection of H. Pylori microorganisms. The kit comes compete with a fixative for air dried cell suspensions and or touch preparation slides. Applications include fine needle aspirations, frozen sections, blood smears, cytological specimens, cytopreps, microorganism detection and bone marrow biopsies. This stain offers results in 15 seconds. The Kit comes complete with 3 solutions and is available in 3 sizes.

RT 26096-25 Differential Quick Staining Kit 250 ml
RT 26096-50 Differential Quick Staining Kit 500 ml
RT 26096-75 Differential Quick Staining Kit 1 gal

arrow11Neat Stain™ Staining Kits

Neat Stain™ Kits are specifically designed for stat procedures and/or laboratories using manual staining methods. Neat Stain™ is available as a rapid three-step differential hematology stain, Gram stain and Trichrome stain staining kit. They are quite simple to use, just immerse your slides sequentially in each reagent for the correct time, air dry and read.

  • Neat – Pre-filled multi-well vessels with self-contained foil sealed
  • Fast – Immerse slides sequentially in each reagent for specified time, air dry and read
  • Convenient – Additional foils for re-sealing containers.

Neat-Stain™ kits are prepared for STAT procedures and /or laboratory using manual staining methods.

technical data sheetNeat Stain GRAM Kit
Neat Stain™ GRAM Kit

This 4-step stain kit is prepared for staining bacteria from cultures or specimens by the differential Gram stain method.

This kit stains similar to the traditional Wright's and Wright-Giemsa stains. Neat Stain® offers the flexibility of the three dip staining sequence in both the stain and counter stain solution.

Kit Contains: 6 Reagent Packs, one Plastic Stand, 6 Re-seal Foils. Kit performs 150 tests.

27100-01 Neat Stain GRAM Kit consists of: kit
GRAM Stain Crystal Violet msds
GRAM Stain Iodine msds
GRAM Stain Decolorizer msds
GRAM Stain Safranin msds
Technical Data SheetNeat Stain Hematology Stain Kit
Neat Stain™ Hematology Stain Kit

This 3-step stain procedure is designed for differentiation of morphological cell types in peripheral blood smears. This kit is also useful in andrology labs staining microorganism and spermatozoa.

Staining characteristics are similar to the Wrights and Wright-Giemsa stains. Neat Stain offers the flexibility of a three dip staining format in both the stain and counter stain solution.

Kit Contains: 6 Reagent Packs, One Plastic Stand, 6 Re-seal Foils. Kit performs 150 tests.

27104-01 Neat Stain Hematology Stain Kit Kit
Technical Data SheetNeat Stain Trichrome Stain Kit
Neat Stain™ Trichrome Stain Kit

This seven-step stain procedure is designed for the staining of stool specimens. Based upon the Wheatley Trichrome technique is a rapid staining procedure providing good results for routine examination. Simplified method, use of mordant prior to staining is not necessary.

Staining characteristics are similar to the traditional Wright's and Wright-Giemsa stains.
Kit Contains: 3 Reagent Packs, One Plastic Stand, 6 Re-seal Foils. Kit performs 75 tests.

Slide Holder sold separately, Cat. #71411-01.

27106-01 Neat Stain Trichrome Stain Kit consists of: kit
Neat Stain Trichrome Chamber 1 msds
Neat Stain Trichrome Chambers 2-3 msds
Neat Stain Trichrome Chamber 4 msds
Neat Stain Trichrome Chamber 5 msds
Neat Stain Trichrome Chambers 6-7 msds

Slide Holderarrow11Slide Holder

Molded from polypropylene. Adjustable to hold 2 to 5 3x1" slides, or 10 slides back-to-back firmly. This Slide Holder works well with all Neat Stain Kits above.

71411-01 Slide Holder each

 

Bacterial, Fungal, and Inclusion Body Stain

Информация для заказа

Наименование ОбъемПроизводствоМетод Кат.Номер
Differential Quick Stain Kit  Electron Microscopy Sciences  26096-25 
   
© ООО «Лабораторная Диагностика»
info@LD.ru   тел.: +7 495 369-20-43