Human p-tau231 (Phospho Tau 231) ELISA Kit

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti p-tau231 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with p-tau231 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of p-tau231 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: EH5058

Alias: p-tau231 ELISA Kit, Phospho Tau 231 ELISA Kit, Microtubule-associated protein tau ELISA Kit, Neurofibrillary tangle protein ELISA Kit, Paired helical filament-tau(PHF-tau ELISA Kit, MAPT ELISA Kit

Species: Human

Range: 7.813-500pg/ml

Sensitivity: 4.688pg/ml

Product Name

Human p-tau231(Phospho Tau 231) ELISA Kit

Alias

p-tau231 ELISA Kit, Phospho Tau 231 ELISA Kit, Microtubule-associated protein tau ELISA Kit, Neurofibrillary tangle protein ELISA Kit, Paired helical filament-tau(PHF-tau ELISA Kit, MAPT ELISA Kit

Catalogue No.

EH5058

Size

48T/96T

Species

Human

UniProt ID

P10636

Sample Type

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Method

Sandwich ELISA, Double Antibody

Detection Wavelength

OD450

Reaction Duration

4 hours

Range

7.813-500pg/ml

Sensitivity

4.688pg/ml

Storage

2-8°C(Sealed), Don't cryopreserve.

Specificity

Specifically binds with p-tau231 , no obvious cross reaction with other analogues.

ELISA Kit Components

Kit Components	Item	Size(48T)	Size(96T)	Storage Condition for Opened Kit
E001	ELISA Microplate(Dismountable)	8×6	8×12	Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002	Lyophilized Standard	1vial	2vial	Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003	Biotin-labeled Antibody(Concentrated, 100X)	60ul	120ul	2-8°C (Avoid Direct Light)
E034	HRP-Streptavidin Conjugate(SABC, 100X)	60ul	120ul
E024	TMB Substrate	5ml	10ml
E039	Sample Dilution Buffer	10ml	20ml	2-8°C
E040	Antibody Dilution Buffer	5ml	10ml
E049	SABC Dilution Buffer	5ml	10ml
E026	Stop Solution	5ml	10ml
E038	Wash Buffer(Concentrated, 25X)	15ml	30ml
E006	Plate Sealer	3 pieces	5 pieces
E007	Product Description	1 copy	1 copy

Required Instruments and Reagents

Microplate reader (wavelength: 450nm)
37°C incubator (CO2 incubator for cell culture is not recommenced.)
Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
Sterile tubes and Eppendorf tubes with disposable tips
Absorbent paper and loading slot
Deionized or distilled water

Информация для заказа

Область использования: ИФА наборы производства Fine Biotech	Производство:	Fine Biotech
	Метод:
	Объем:	96T
	Кат. номер:	EH5058
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Human p-tau231 (Phospho Tau 231) ELISA Kit. Примечание: