| General |
| Detection modes |
Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa®, Luminescence, UV-Visible Absorbance |
| Read methods |
End point, kinetic, spectral scanning, well-area scanning |
| Microplate types |
6- to 1536-well plates (luminescence 1- to 384-well plates) PCR plates |
| Other labware supported |
Take3™ Micro-Volume Plate |
| Temperature control |
4-Zone™ incubation to 65 °C; variation +0.2 °C at 37 °C |
| Shaking |
Linear |
| Software |
Gen5™ Data Analysis Software |
| Automation |
BioStack™ and 3rd party automation compatible |
| AlphaScreen/AlphaLISA |
| Light source |
Tungsten halogen |
| Sensitivity |
100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate |
| Dynamic range |
>6 decades |
| Reading speed |
2 minutes (96-well plate) |
| Detection system |
PMT |
| Absorbance |
| Light source |
Xenon flash |
| Wavelength selection |
Monochromator |
| Wavelength range |
200 - 999 nm, 1 nm increment |
| Bandwidth |
2.4 nm |
| Dynamic range |
0 - 4.0 OD |
| Resolution |
0.0001 OD |
| Pathlength correction |
Yes |
| Monochromator wavelength accuracy |
+2 nm |
| Monochromator wavelength repeatability |
+0.2 nm |
| OD accuracy |
<1% at 2 OD <3% at 3.0 OD |
| OD linearity |
<1% from 0 to 3.0 OD |
| OD repeatability |
<0.5% at 2.0 OD |
| Stray light |
0.03% at 230 nm |
| Reading speed |
96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds |
| Fluorescence Intensity |
| Light source |
Tungsten halogen High energy DPR Xenon flash (optional) |
| Detector |
PMT |
| Wavelength selection |
Deep blocking bandpass filters/dichroic mirrors |
| Wavelength range |
300 - 700 nm with tungsten lamp (850 nm option) 200 - 700 nm with xenon lamp (850 nm option) |
| Dynamic range |
>6 decades |
| Sensitivity |
Top: Fluorescein 1 pM (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate)
Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) |
| Bandwidth |
Filter dependent |
| Reading speed |
96 wells: 11 seconds 384 wells: 22 seconds 1536 wells: 42 seconds |
| Luminescence |
| Wavelength range |
300 - 700 nm |
| Dynamic range |
>6 decades |
| Sensitivity |
10 amol ATP (flash) 100 amol ATP (glow) |
| Detection system |
PMT |
| Fluorescence Polarization |
| Light source |
Tungsten halogen High energy DPR Xenon flash (optional) |
| Wavelength selection |
Deep blocking filters/dichroic mirrors |
| Wavelength range |
400 - 700 nm (320 - 850 nm option) |
| Sensitivity |
3 mP at 1 nM fluorescein |
| Time-Resolved Fluorescence |
| Light source |
High Energy DPR Xenon flash |
| Wavelength selection |
Deep blocking filters/mirrors |
| Wavelength range |
200 - 700 nm (850 nm option) |
| Sensitivity |
Europium 60 fM (12 amol/well 96-well plate; 6 amol/well 384-well plate) |
| Reagent Dispensers |
| Number |
2 syringe pumps |
| Supported labware |
6- to 96-well microplates |
| Dead volume |
1 mL, 100 µL with back flush |
| Dispense volume |
5 - 1000 µL in 1 µL increment |
| Dispense accuracy |
+1 µL or 2% |
| Dispense precision |
<2% at 50 - 200 µL |
| Physical Characteristics |
| Power |
250 Watts max. |
| Dimensions |
17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm) |
| Weight |
60 lbs (27 kg) |