ООО «Лабораторная Диагностика»
ООО «Лабораторная Диагностика»  
 Главная   Новости   О компании  Каталог продукции и цены   Оформить заказ   Контакты 
 
  
Каталог продукции
  Гематология  
  Гемостаз  
  Иммуногематология  
  Иммуногистохимия
  Иммуноферментный анализ (ИФА)
  Иммунохимия  
  Клеточные технологии  
  Клиническая биохимия
  Коагулометрия  
  Микробиология  
  Мультиплексный анализ  
  Оборудование для биохимии и
  молекулярной биологии
  Общелабораторное оборудование
  Онкология  
  Пищевая промышленность  
  Программное обеспечение  
  Проточная цитометрия
  ПЦР-лаборатория
  Рекомбинантные белки
  Репродуктивные технологии
  Сортировка клеток
  Спектрофотометрия  
  Стволовые клетки (Stem Cells)
  Трансплантология
  Цитогенетическая диагностика
  Экспресс-лаборатория  

 
/ Каталог / Молекулярная диагностика / Экстракция нуклеиновых кислот / Реагенты для очистки и выделения ДНК и нуклеиновых кислот / Выделение РНК и иРНК

Набор mirVana™ miRNA Isolation Kit, with phenol

Набор mirVana™ miRNA Isolation Kit (+4 °C)

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.

Features of this kit include:

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107 cultured cells or 0.5–250 mg tissue.

Enrich for small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ~200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

Accessory product
The mirVana™ miRNA Isolation Kit without Phenol is available for customers concerned about the cost of shipping organic chemicals such as Acid Phenol:Chloroform, which is included in the standard kit.
For Research Use Only. Not for use in diagnostic procedures.
 

Спецификация

Sample Type (General): Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: microRNA Analysis
Final Product: Total RNA, Transcriptome RNA, micro RNA, siRNA, snRNA
Isolation Technology: Organic Extraction, Spin Column (Glass Fiber Filter)
Product Line: mirVana™, Ambion™
Product Size: 40 preps
Starting Material (Amount): Up to 10^7 cells, Up to 250 mg tissue

Содержимое и хранение

• 30 ml miRNA Wash Solution I (room temperature)
• 50 ml Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 ml Lysis/Binding Buffer (4°C) 
• 10 ml miRNA Homogenate Additive (4°C) 
• 100 ml Acid-Phenol:Chloroform (4°C) 
• 1.4 ml Gel Loading Buffer II (-20°C) 
• 5 ml Elution Solution (-20°C, 4°C, or room temperature)

Информация для заказа

Область использования:Производство:Ambion
Метод: 
Объем:40 preps 
Кат. номер:AM1560
Цена (с НДС 18%):по запросуВ корзину
Наименование: Набор mirVana™ miRNA Isolation Kit, with phenol / Набор mirVana™ miRNA Isolation Kit (+4 °C).
Примечание:
   
© ООО «Лабораторная Диагностика»,  info@LD.ru
телефоны: +7 (495) 461-67-60, +7 (499) 348-25-72