ДНК-зонды для онкогематологии ON C-MYC (8q24) / SE 8, цвета флуорохромов - red/green
Poseidon™ Repeat Free™ C-MYC (8q24) & SE 8 Control probe
Introduction: C-MYC gene activation (enhanced expression and/or amplification) may result from chromosomal duplication as well as translocation. Amplification of C-MYC has been described in many types of solid tumours, such as breast, cervical and colon cancers, as well as in myeloma, non-Hodgkin's lymphoma, gastric adenocarcinomas and ovarian cancer. Multiple copies of the gene may be evidenced in homogeneously staining chromosomal regions and in double minutes.
Intended use: The C-MYC (8q24) specific DNA Probe is optimized to detect copy numbers of the C-MYC gene region at 8q24. The Chromosome 8 Satellite enumeration (SE) probe is included to facilitate chromosome identification. Note: This probe should not be used to detect translocations involving C-MYC. The probe is recommended to be used in combination with a Poseidon FISH Kit providing necessary reagents to perform FISH (KBI-60002, KBI-60003 or KBI-60001) for optimal results.
Critical region 1 (red): The C-MYC (8q24) specific DNA probe is direct-labeled with PlatinumBright550.
Control region 2 (green): The SE 8 control DNA probe is direct-labeled with PlatinumBright495.
Reagent: Poseidon probes are direct-labeled DNA probes provided in a ready-to-use format. Apply 10 µl of probe to a sample area of approximately 22 x 22 mm. Please refer to the Instructions for Use for the entire Poseidon FISH protocol. Poseidon Repeat Free probes do not contain Cot-1 DNA. Hybridization efficiency is therefore increased and background, due to unspecific binding, is highly reduced.
Interpretation: The C-MYC (8q24) probe is designed as a dual-color assay to detect amplifications at 8q24. Amplification involving the C-MYC gene region at 8q24 will show three or more red signals, while the control at the chromosome 8 centromere will provide 2 signals (3R2G) Two single color red (R) and green (G) signals will identify the normal chromosomes 8 (2R2G)
Normal Signal Pattern Amp(8q24)
Expected Signals 2R2G 3+R2G
References: Bentz, M et al, 1995, Blood, 85; 3610-3618 Persons DL et al, 1997, Mod Pathol. 10; 720-727
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|Наименование: ДНК-зонды для онкогематологии ON C-MYC (8q24) / SE 8, цвета флуорохромов - red/green.|
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