Paraformaldehyde to Picric

Paraformaldehyde, EM Grade, Purified

(Polyoxymethylene)
OH(CH₂O)_n H   F.W. Average: 600   CAS #30525-89-4

Paraformaldehyde is the condensation product of methylene glycol, a substance not so far isolated in its pure form; it is a white solid with a formaldehyde content between 78 and 98% and is supplied either as a Prill or Granular. All forms contain more than 95.5% paraformaldehyde content. The Prill and Granular forms are small, free-flowing, dust-free beads, the most desired by EM users. A fast-penetrating EM fixative used in combination with Glutaraldehyde, Acrolein, and Osmium Tetroxide. Karnovsky, M.J., J. Cell Biol., 27, 137A (1965). Hayat, M.A. Principles & Tech., of EM (Univ. Park Press), 68 (1981).

Technical Tip

Paraformaldehyde Static Jumps

 

Paraformaldehyde Aqueous Solution -16% (Formaldehyde Aqueous Solution, 16%)

CAS #30525-89-4
Prepared from Paraformaldehyde crystal, 16% aqueous solution, methanol free. Microfiltered and stored in pre-scored ampoules.

RT	15700	16% Paraformaldehyde (formaldehyde) aqueous solution		10 ml
RT	15710	16% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	15710-S	16% Paraformaldehyde (formaldehyde) aqueous solution		100 ml bottle
RT	15711	16% Paraformaldehyde (formaldehyde) aqueous solution		10 bxs/lot
RT	15712	10% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	15712-S	10% Paraformaldehyde (formaldehyde) aqueous solution		100 ml
RT	15713	20% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	15713-S	20% Paraformaldehyde (formaldehyde) aqueous solution		100 ml
RT	15714	32% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	15714-S	32% Paraformaldehyde (formaldehyde) aqueous solution		100 ml

We can prepare in accordance to your special requirements (any concentration desired). Call for more information.

See Formaldehyde Solution, for other standard concentrations.

In addition to our complete line of Formaldehyde solutions shown above we have now added to the line.

Since the DOT (Department of Transportation) has changed the definition of what a non dangerous solution of formaldehyde is to under 10%, we are now offering an 8% and a 4% solution. This will allow us to ship overnight as well as overseas without any hazardous charges saving our customers a good deal of money.

The quality of our 8% and 4% aqueous Paraformaldehyde solution is as good as all of the other concentrations that we offer.

RT	157-4	4% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	157-4-100	4% Paraformaldehyde (formaldehyde) aqueous solution		100 ml
RT	157-8	8% Paraformaldehyde (formaldehyde) aqueous solution		10x10 ml
RT	157-8-100	8% Paraformaldehyde (formaldehyde) aqueous solution		100 ml

Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative

Ready To mix. Kit consists of: 2 x 10 ml ampoules 16% Formaldehyde 1 x 10 ml ampoules 50% Glutaraldehyde1x50 ml 0.2M Sodium Phosphate Buffer.

Fixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

Paramat and Paramat Extra

Please see listing in Embedding Media for Histology and Light Microscopy.

Tissue-TEK Paraffin

Please see listing in Embedding Media.

Paraffin Wax, ParaPro Blue™

A specialized wax which consists of light blue pellets used specifically for small biopsy specimens, as well as for general use with all other tissues.

The ParaPro Blue offers better visualization of the tissue and allows for sectioning as low as 3µm thick. The melting point of the wax is 56°C and offers perfect ribbon continuity.

Paraplast Embedding Medium

Paraplast X-tra

Handy pellet form melts rapidly. Melting Point 53-54°C. Cuts to 2 micron thickness with exceptional ribbon continuity. Extra compression resistance provides total support of tissues.

Paraplast Plus

Reduces infiltration time by one-third. Cuts to 2 micron thickness with excellent ribbon continuity. Convenient pellet form melts rapidly. Melting point 56°C. Double filtered. No need for filtration before use.

Paraplast Medium

Handy pellet form melts rapidly. Melting Point 56°C.
Cuts to 4 micron thickness with excellent ribbon continuity – no Crumbling or cracking.
Double filtered. No need for filtration before use.

Paraffin Waxes

For a continuation of our Paraffin Waxes, please see listing in Embedding Media.

Articles of Interest

Re-Embedding of Paraffin Embedded Specimens in Resin

Paraffin - Peel-A-Way® Embedding Wax

For our complete line of Embedding Waxes, please see Embedding Media for Histology and Light Microscopy.

PEG- GMA/PEG Embedding Kit

Please see listing in Embedding Media Kits.

Parlodion (Pyroxylin Purified Strips)

C₁₂H₁₆N₄O₁₈   F.W. 504.28   CAS #9004-70-0
Highly purified from cellulose nitrate; specially prepared for embedding tissue for sectioning and for preparing semi-permeable membranes.

Parlodion 2% in Amyl Acetate

See Collodion, 2%.

Pepsin Reagent, Ready To Use

Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use:Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml

Periodic Acid, Reagent

H₅IO₆   F.W. 227.96   CAS #10450-60-9

Used as a Fixative: Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.
Used widely as a Negative Stain:

• As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
• Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
• Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
• Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
• Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.

Periodic Acid Solutions

Please see listing in Reagents for Histology.

Permount™ Mounting Medium

Please see listing in Adhesives and Mountants.

PHEM Buffer

EMS is proud to announce and release of our newest Buffer for EM PHEM.

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies.  Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

References:

1. Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
2. Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers

Academic Editor: James Reimer, published online March 31, 2016

Phenol Red

(Phenolsulfonphthalein)
C₁₉H₁₄O₅S   F.W. 354.38   CAS #143-74-8
A standard indicator for preparing buffers, pH range 6.4-8.2.

Phenol Red Sodium Salt

(Phenolsulfonphthalein Sodium Salt)
C₁₉H₁₃NaO₅S F.W. 376.37 CAS #34487-61-1
A standard indicator for preparing buffers, pH range 6.8-8.2.

(PMSF), Phenylmethylsulphonyl Fluoride

C₆H₅CH₂SO₂F   F.W. 174.20   CAS #329-98-6

Phosphate Mixed Sodium Salts 0.2 M Buffer

Please see listing in Prepared Buffers.

Phloxine B, Certified, C.N. #DcPh-6

(Acid Red 92; Cyanosine; Eosin 10B; Phloxine TA, N, BP Super, RB, TB, or BB)
C₂₀H₂O₅Cl₄Br₄Na₂   F.W. 829.66   CAS #18472-87-2
Solubility: 10.5% Water; 5.0% Alcohol; 9.0% Cellosolve; 4.5% Glycol; 0.0% Xylene

Phosphomolybdic Acid, Crystal, Reagent, A.C.S.

(dodeca-Molybdophosphoric Acid)
H₃PO₄·12(MoO₃)·24H₂O   F.W. 2257.6   CAS #51429-74-4

Phosphomolbdic Acid Solutions

Please see listing in Reagents for Histology.

Phosphotungstic Acid, Crystal, Reagent, Highest Purity

(Tungstophosphoric Acid)
H₃[P(W₃O₁₀)₄]   aq F.W. 2880.17+aq   CAS #12501-23-4
Specifications:

Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.

Used widely as a Negative Stain:

• As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
• Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
• Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550.,Farragiana, T., and Marinozzi, V. (1979).
• Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
• Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.

RT

19500

Phosphotungstic Acid, Crystal, Reagent, Highest Purity

100g

Phosphotungstic Acid Solutions

Please see listing in Reagents for Histology.

Picric Acid, Reagent, Crystal, A.C.S.

(2,4,6-Trinitrophenol)
C₆H₂(NO₂) ₃OH FW 229.11 CAS #88-89-1
A.C.S. Specifications:

Наименование	Объем	Производство	Метод	Кат.Номер
16% Paraformaldehyde Aqueous Solution EM Grade, methanol free, 10x10 ml		Electron Microscopy Sciences		15710