Rabbit Anti Human RAF1 PolyClonal AntibodyСпецификация| Объем | 100 мкл (100 мкг) | | Синонимы | RAF1,CRAF,NS5,Raf-1,c-Raf | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P04049 | | Иммуноген | C term -peptide of human c-Raf | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:2000 | | Примечание | A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway . Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 . p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity . Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf . Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively . While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed . Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf . The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma . Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events . | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity purification | | Области исследований | Cancer,Cell biology,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts of mouse brain and mouse heart tissue, using c-Raf antibody.
 | | | |
Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | | | Объем: | 100 мкл (100 мкг) | | Кат. номер: | CSB-PA019284KA01HU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: Rabbit Anti Human RAF1 PolyClonal Antibody.
Примечание: дополнительная информация (на английском языке). |
|
