Антитела Anti-Syndecan-1 (CD-138) antibody [EPR6454], кроличьи моноклональные, RabMAb

Anti-Syndecan-1 antibody [EPR6454]

Product nameAnti-Syndecan-1 antibody [EPR6454]
See all Syndecan-1 primary antibodies
Description
Rabbit monoclonal [EPR6454] to Syndecan-1
SpecificityBased on negative staining of mouse and rat spleen and stomach tissues in IHC-P we believe this antibody is unsuitable for IHC-P with mouse and rat. We believe the antibody is suitable for Western blot with mouse and rat based on data from brain, heart, kidney, and spleen lysates. Please contact our Scientific Support team for more information.
Tested applicationsICC/IF, WB, IHC-Pmore details
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Syndecan-1 aa 1-300 (extracellular).
Database link: P18827
Positive control
- Human colon and cervical carcinoma tissues; Fetal kidney, HeLa, PC12, Raji, Ramos and BxPC-3 cell lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Produced using Abcam’s RabMAb® technology. RabMAb®technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

FormLiquid
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
ConcentrationBatch dependent within range: 100 µl at 1.086 - 1.123 mg/ml Batch dependent within range: 10 µl at 1.086 - 1.123 mg/ml

Find concentration of your lot
PurityProtein A purified
ClonalityMonoclonal
Clone numberEPR6454
IsotypeIgG
Research areas

Associated products

Positive Controls
Related Products
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Applications

Our Abpromise guarantee covers the use of ab128936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		Use a concentration of 2 µg/ml.
WB		1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
IHC-P		1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/500 - 1/1000.

Application notesIs unsuitable for Flow Cyt or ICC.

Target

FunctionCell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
Sequence similaritiesBelongs to the syndecan proteoglycan family.
Cellular localizationMembrane.
Information by UniProt
Database links
- Entrez Gene: 6382 Human
- Entrez Gene: 20969 Mouse
- Entrez Gene: 25216 Rat
- Omim: 186355 Human
- SwissProt: P18827 Human
- SwissProt: P18828 Mouse
- SwissProt: P26260 Rat
- Unigene: 224607 Human
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Alternative names
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
see all

Anti-Syndecan-1 antibody [EPR6454] images

Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/10000 dilution (purified)

Lane 1 : Ramos whole cell lysate
Lane 2 : Raji whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size : 32 kDa
Observed band size : 35,100 kDa (why is the actual band size different from the predicted?)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

All lanes : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/5000 dilution (purified)

Lane 1 : HeLa whole cell lysate
Lane 2 : PC-12 whole cell lysate
Lane 3 : Mouse heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size : 32 kDa
Observed band size : 35,100 kDa (why is the actual band size different from the predicted?)

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (ab128936)

Immunohistochemical staining of paraffin embedded human colon with purified ab128936 at a working dilution of 1/8000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Syndecan antibody [EPR6454] (ab128936)

Lanes 1 - 5 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution (Unpurified)
Lane 6 : Anti-Syndecan-1 antibody [EPR6454] (ab128936) at 1/1000 dilution

Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : BxPC-3 cell lysate
Lane 5 : Fetal kidney lysate
Lane 6 : PC12 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 32 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65-100 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan antibody [EPR6454] (ab128936)

Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan antibody [EPR6454] (ab128936)

Unpurified ab128936, at a 1/500 dilution, staining Syndecan in paraffin-embedded Human colon tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Syndecan antibody [EPR6454](ab128936)

Unpurified ab128936 showing positive staining in Normal skin tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Syndecan antibody [EPR6454](ab128936)

Unpurified ab128936 showing positive staining in normal tonsil tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Syndecan antibody [EPR6454](ab128936)

Unpurified ab128936 showing positive staining in Plasmacytoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Syndecan antibody [EPR6454](ab128936)

Unpurified ab128936 showing negative staining in Skeletal muscle tissue.

References for Anti-Syndecan-1 antibody [EPR6454] (ab128936)

ab128936 has not yet been referenced specifically in any publications.

Информация для заказа

Область использования: Прочие реагенты для иммуногистохимии	Производство:	Abcam
	Метод:
	Объем:	100 мкл
	Кат. номер:	ab128936
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Антитела Anti-Syndecan-1 (CD-138) antibody [EPR6454], кроличьи моноклональные, RabMAb / Anti-Syndecan-1 antibody [EPR6454]. Примечание: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Дополнительная информация (на английском).