ИФА набор Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kitRat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Product Details
Target NameAlternative NamesAbbreviationUniprot No.SpeciesSample TypesDetection RangeSensitivityAssay TimeSample VolumeDetection WavelengthResearch AreaAssay PrincipleMeasurementPrecisionLinearityRecoveryTypical DataTroubleshootingand FAQsStorage
| matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| Mmp2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit |
| MMP2 |
| P33436 |
| Rattus norvegicus (Rat) |
| serum, plasma, cell culture supernates, tissue homogenates |
| 0.625 ng/mL-40 ng/mL |
| 0.156 ng/mL |
| 1-5h |
| 50-100ul |
| 450 nm |
| Cancer |
| quantitative |
| Sandwich |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:10 |
Average % |
93 |
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| Range % |
90-93 |
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| 1:20 |
Average % |
89 |
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| Range % |
84-94 |
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| 1:40 |
Average % |
96 |
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| Range % |
91-100 |
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| The recovery of rat MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
87-96 |
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| EDTA plasma (n=4) |
103 |
95-108 |
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| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.194 |
2.283 |
2.239 |
2.093 |
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| 20 |
1.804 |
1.959 |
1.882 |
1.736 |
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| 10 |
1.280 |
1.386 |
1.333 |
1.187 |
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| 5 |
0.891 |
0.870 |
0.881 |
0.735 |
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| 2.5 |
0.455 |
0.464 |
0.460 |
0.314 |
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| 1.25 |
0.297 |
0.307 |
0.302 |
0.156 |
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| 0.625 |
0.228 |
0.225 |
0.227 |
0.081 |
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| 0 |
0.142 |
0.150 |
0.146 |
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| ELISA kit FAQs |
| Store at 2-8°C. Please refer to protocol. |
Спецификация| Специфичность | Rattus norvegicus (Rat) | | Тип образца | serum, plasma, cell culture supernates, tissue homogenates | | Sensitivity | 0.156 ng/mL | | Detection Range | 0.625 ng/mL-40 ng/mL | | Assay Principle | quantitative | | Measurement | Sandwich | | Assay Time | 1-5h | | Объем образца | 50-100ul | | Длина волны | 450 nm | | Область исследований | Cancer | | Abbreviation | MMP2 | | Uniprot No# | P33436 | | Target Name | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) | | Alias | CLG4, CLG4A, MMP-II, MONA, TBE-1, collagenase type IV-A|matrix metalloproteinase 2|matrix metalloproteinase-II|neutrophil gelatinase | | Варианты фасовки | 96T,5×96T,10×96T | | Инструкция | ссылка | | Ссылка на страницу на сайте производителя | ссылка | | | |
Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | | | Объем: | 96 | | Кат. номер: | CSB-E07411r | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: ИФА набор Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit / Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit.
Примечание: дополнительная информация (на английском языке). |
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