HUMAN ANTI-PEG IGG ELISA (Life Diagnostics)
INTRODUCTION
Attachment of polyethylene glycol (PEG) chains to therapeutic
biologic agents, a process referred to as PEGylation, prolongs the
circulating half-life of the modified protein by slowing proteolytic
degradation and by masking it from the immune system. However, it
has been reported that repeated injections of PEGylated proteins can
induce anti-PEG antibodies that increase the rate of clearance and
decrease drug efficacy (accelerated blood clearance, or ABC
phenomenon). To aid research in this important area, we have
developed a human anti-PEG IgG ELISA kit.
PRINCIPLE OF THE ASSAY
The assay uses immobilized mono-mPEGylated BSA (20 kDa PEG
chain) as the capture antigen (coated on microtiter wells) and
horseradish peroxidase (HRP) conjugated anti-human/monkey IgG
monoclonal antibody (LDI clone 6D11-21
) for detection. Serum and
plasma samples are diluted and incubated alongside standards in the
microtiter wells for 1 hour. The wells are subsequently washed. HRP
conjugate is added and incubated for 45 minutes. Anti-PEG IgG
molecules are thus sandwiched between immobilized PEG and the
detection antibody conjugate. The wells are then washed to remove
unbound HRP-labeled antibodies. TMB reagent is added and
incubated for 20 minutes at room temperature. This results in the
development of a blue color. Color development is stopped by the
addition of Stop Solution, changing the color to yellow. Optical density
is measured spectrophotometrically at 450 nm. The concentration of
anti-PEG IgG is proportional to the absorbance at 450 nm and is
derived from a standard curve.
Информация для заказа
| Область использования: | Производство: | --- |
| Метод: | |
| Объем: | |
| Кат. номер: | PEGG-20 |
| Цена (с НДС 20%): | по запросу | В корзину  |
Наименование: HUMAN ANTI-PEG IGG ELISA (Life Diagnostics).
Примечание: |
